Genetic Engineering Escherichia Coli - 1789 Words

Genetic engineering is the deliberate modification of the characteristics of an organism by manipulating its deoxyribonucleic acid (DNA). Bacteria possess plasmids which can be used as vectors for bacterial transformation. The plasmids can be altered to include donor DNA thus altering the physiological or physical characteristics of the bacterium. Escherichia coli have been found to be the most effective bacterium in genetic transformation and heterologous expression of human genes or proteins. It was hypothesised that Escherichia coli HB101 K12 could be transformed with a pGLO plasmid to express the genes encoded for green fluorescent protein (GFP), the bla gene for ampicillin resistance and the araC gene a regulatory protein in facilitating transcription and therefore the transformation efficiency for this bacteria could be calculated. Bacillus megaterium, E.coli B strain, Erwinia carotovora, Pseudomonas fluorescens, Staphylococcus epidermidis and Streptococcus lactis will also be subjected to transformation with the pGLO plasmid to collect transformation efficiency data for comparison against Escherichia coli HB101 K12. Escherichia coli HB101 K12 were found to have been successfully transformed with a transformation efficiency falling within the transformation protocol of 8.0 x 102 and 7.0 x 103. The incidence of Escherichia coli HB101 K12 on the LB/amp and LB/amp/ara pGLO+ plates indicated that the bacterial colonies present showed resistance to the ampicillinShow MoreRelatedBearing Light On Gene Expression : Streamlining Light Controllable Escherichia Coli1041 Words   |  5 PagesBearing Light on Gene Expression: Streamlining Light Controllable Escherichia coli Two-Component Transduction Systems Antonio Torres November 16, 2014 Advanced Biochemistry and Molecular Biology CHEM 4360/5383 Abstract Optogenetics is a rapidly evolving technology that, using a combination of optical methods and genetics, is capable of producing high resolution information on specific events of interest within biological systems, whether they be freely moving organisms or not (Diesseroth, 2011)Read MoreLara Guvelioglu. Bi 108 E2. Nahomie Rodriguez-Sastre. 04/13/17.1632 Words   |  7 PagesRodriguez-Sastre 04/13/17 Bacteria Escherichia coli’s Genetic Transformation Using Vector Plasmid DNA pGLO Abstract The field of biotechnology involves the concept of genetic engineering, altering the DNA/genetic material of an organism using information from a different one. The process in which bacteria can obtain this manipulated genetic information from another source is called genetic transformation. The goal of this experiment was to genetically transform Escherichia coli bacteria’s DNA by insertingRead MoreTransformation Of Escherichia Coli With pGLO Plasmid1560 Words   |  7 Pagesï » ¿Transformation Of Escherichia Coli With pGLO Plasmid April 24, 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait, in this case antibiotic resistance. 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These concentrations are namely, 5  µl of distilled water, which acts as the control in this experiment; 2.5  µl of undiluted plasmid DNA; 1.0 µl of undiluted plasmid DNA; andRead MoreBacterial Transformation ( Escherichia Coli )1050 Words   |  5 Pages Bacterial Transformation (Escherichia coli) Kadian Jones Dr. F. Redway BIO 104- Lab 3 November 11, 2014 Introduction According to Medicine.net (2012), genetic transformation is a process by which the genetic material carried by an individual cell is altered by the incorporation of foreign/exogenous DNA into its genome. Competent cells are able to accept DNA presented by experimental influence or manipulation, and the application of genetic engineering with bacteria can aid in theRead MoreIdentifying An Unknown Bacterium Broth Culture1880 Words   |  8 Pagestyphimurium and Escherichia coli. The next test I did was a phenol red lactose (PRL) broth and a streak on an EMB plate, both incubated for 48 hours at 37 degrees Celsius. 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Finally, E. coli cells will express a dual plasmid system to control population growthRead MoreEssay on Bacteria and Viruses1259 Words   |  6 Pagesthat are good, such as the ways that make cheese and yogurt, or clean up oil spills. They may also be commensals, such as Escherichia coli. The positive effects of viruses are less obvious. Viruses can function as vaccines for similar but more harmful diseases, be scientifically interesting and broaden our understanding of biology, or have applications in genetic engineering as transfer vectors (â€Å"Gene transfer vector†, 1999.) This is because of the way that viruses infect cells and replicate. Viruses